This paper examines the impact of temperature, pressure, and blade thickness on the stresses experienced by impeller blades during operation. The impeller is subject to a range of stresses arising from thermal, fluid, and mechanical factors that can cause deformation if they exceed certain thresholds. The study focuses on an impeller supplied by HAUS Centrifuge Technologies, which suffered damage while operating at 33000 rpm. Six blade thickness offsets, ranging from 0.05 mm to 0.15 mm, were analyzed structurally, and the Von-Mises stresses were compared to the impeller material yield strength. The impeller with the lowest stress, at 197.43 MPa, was chosen for fluid-structure interaction analysis. The impeller was then manufactured and tested for performance using ISO 5389 standard in HAUS test facility. The CFD results indicated that the polytropic efficiency of the thickened impeller increased to 86.57%, compared to the original impeller polytropic efficiency of 75.8%. However, the volumetric flow decreased from 4211.3 m3 per hour to 3658.3 m3 per hour when using "the thicker impeller". The data collected supports the conclusion that an increase in blade thick-ness can help to reduce the stresses acting on the blade.