The paper tests the changes in the pH value of the anolyte and catholyte. The 3-D multi-phase 3-D multi-current conductivity values analyze the electricity generation process and energy utilization of the microbial fuel cell (AMFC) and provide a theory for improving the AMFC following the performance. The test results show that with the operation of AMFC, the pH value of the anolyte and the 3-D multi-flow conductivity show a downward trend, the pH value of the catholyte and the 3-D multi-flow conductivity show an upward trend, and the ratio of the pH value of the catholyte the pH value of the anolyte is about 0.30-0.50 higher, and the average 3-D multi-current conductivity of the anolyte and catholyte does not change much. When AMFC operates stably, the internal ohmic resistance is 29.69 Ω, the limiting current is 2.69 mA, the maximum output power is about 0.8 mW, and the corre­sponding internal resistance is about 95.72 Ω. The mass transmission of potassium ferricyanide is the limiting factor of limiting current. Numerical analysis of 3-D multi-phase flow found that other microorganisms consume 91.1% of the glucose in AMFC anolyte, and only 8.9% of the glucose is used for power generation. The 88.5% of the energy of the glucose used for power generation is converted into other forms of energy, only 11.5% of the energy is converted into electricity.