The development of a two zone k-ε turbulence model for the cycle-simulation software is presented. The in-cylinder turbulent flow field of internal combustion engines plays the most important role in the combustion process. Turbulence has a strong influence on the combustion process because the convective deformation of the flame front as well as the additional transfer of the momentum, heat and mass can occur. The development and use of numerical simulation models are prompted by the high experimental costs, lack of measurement equipment and increase in computer power. In the cycle-simulation codes, multi zone models are often used for rapid and robust evaluation of key engine parameters. The extension of the single zone turbulence model to the two zone model is presented and described. Turbulence analysis was focused only on the high pressure cycle according to the assumption of the homogeneous and isotropic turbulent flow field. Specific modifications of differential equation derivatives were made in both cases (single and two zone). Validation was performed on two engine geometries for different engine speeds and loads. Results of the cyclesimulation model for the turbulent kinetic energy and the combustion progress variable are compared with the results of 3D-CFD simulations. Very good agreement between the turbulent kinetic energy during the high pressure cycle and the combustion progress variable was obtained. The two zone k-ε turbulence model showed a further progress in terms of prediction of the combustion process by using only the turbulent quantities of the unburned zone.