THERMAL SCIENCE
International Scientific Journal
THERMOKINETICS AND RHEOLOGY OF AGAROSE GEL APPLIED TO BIOPRINTING TECHNOLOGY
ABSTRACT
The paper presents new results on the study of thermokinetics of gel system based on agarose in the process of transition from solution to gel and opposite. This issue is extremely relevant, since the stability and predictability of thermophysical and rheological properties in such transformations, especially in the presence of components of the nutrient medium and immobilized microorganisms, come to the fore in terms of design and selection of modes of operation of the printing device promising 3-D bioprinters, as well as the system of preparation and storage of the presence of the hysteresis effect, both from the point of view of the kinetics of gel formation and from the point of view of the dependence of rheological properties on temperature, at different concentrations of modifying components, is shown. The obtained results allow to draw a conclusion about the possibility of using the scheme with preliminary preparation of the initial biogel for the implementation of bioprinting technology based on agarose, and to recommend the obtained values for modeling the operating modes of devices of this type.
KEYWORDS
PAPER SUBMITTED: 2019-11-01
PAPER REVISED: 2019-11-14
PAPER ACCEPTED: 2019-11-16
PUBLISHED ONLINE: 2019-12-22
THERMAL SCIENCE YEAR
2020, VOLUME
24, ISSUE
Issue 1, PAGES [347 - 353]
- Amy L Paguirigan, David J Beebe. Protocol for the fabrication of enzymatically crosslinked gelatin microchannels for microfluidic cell culture. Nature Protocols volume 2, pp. 1782 - 1788 (2007)
- Yan Wang, et al. Diffusion characteristics of agarose hydrogel used in diffusive gradients in thin films for measurements of cations and anions. Analytica Chimica Acta, Volume 945, 2016, pp. 47-56]
- N.A. Hadjiev, B.G. Amsden. An assessment of the ability of the obstruction-scaling model to estimate solute diffusion coefficients in hydrogels. Journal of Controlled Release. Volume 199, 2015, pp. 10-16
- A. Paajanen, T. Pinomaa, H. Pajari, S. Metsä-Kortelainen, P. Lahtinen, A. Nurmela Modeling the 3D Printing of Nanocellulose Hydrogels, NAFEMS Exploring the Design Freedom of Additive Manufacturing through Simulation - Helsinki, Finland, 2016
- M. Stanton, J. Samitier and S. Sanchez Bioprinting of 3D hydrogels, Lab Chip, 2015, 15, 3111-3115
- Y. Zhang, K. Julio A. Kamyar, M. Bakht, J. Yang 3D Bioprinting for Tissue and Organ Fabrication, Annals of Biomedical Engineering, vol. 45, Issue 1, pp 148-163
- R. Fan, M. Piou, E. Darling, Bio-printing cell-laden Matrigel-agarose constructs, Journal of Biomaterials Applications, J Biomater Appl. 2016 Nov;31(5):684-692. 2016
- S. Arnott, A. Fulmer W.E.Scott, I.Dea, R.Moorhouse, D.A.Rees The agarose double helix and its function in agarose gel structure, Journal of molecual biology, vol. 90(2), pp. 269-272
- B. Pokusaev, A. Vyazmin, N. Zakharov, S. Karlov, D. Nekrasov, V. Reznik, D. Khramtsov The effect of bioresorbable additives and micro-bioobjects on gel formation, stabilization and thermophysical properties, Thermal Science, vol. 23, 2019
- I. Korotyaev, S. V. Babichev. "Medical Microbiology, immunology and Virology", St. Petersburg, Spetslit, 2017, 1490 p (in Russian)
- V. Normand, L. Didier, E. Amici, Kevin P. P. Pierre New Insight into Agarose Gel Mechanical Properties, Biomacromolecules 2000, 1, 4, pp. 730-738
- Demchenko D. V., Pozharitskaya O. N., Shikov A. N. and others. Rheological studies of agar gels for the creation of soft capsule shells / / Chemico-pharmaceutical journal. 2013. T. 47. No. 10. pp. 40-42. (in Russian)
- Shipunov, B. P., Koptev V. E., Markin, V. I. the peculiarities of rheological properties of solutions of agar-agar. Chemistry of plant raw materials. 2018. No. 1. pp. 53-60. (in Russian)
- B. Pokusaev, S. Karlov, D. Nekrasova, N. Zakharov, D. Khramtsov, V. Reznik, A. Vyazmin, N. Shumova Agarose Gels with Bioresorbable Additives: the Kinetics of the Formation, Structure, Some Properties, Chemical Engineering Transactions, vol. 74, 2019
- K. Madlener, B. Frey and H. K. Ciezki Generalized reynolds number for non-newtonian fluids, Progress in Propulsion Physics 1 (2009) 237-250
- W. Carval, M. Djabourov Physical gelation under shear for gelatin gels, Rheologica Acta (1997) vol. 36(6), pp. 591-609