The optimal refrigeration temperature control and pre-cooling system optimization of fruits and vegetables in the process of cold chain transportation are of paramount importance in ensuring product preservation, quality, and safety. This paper focuses on the changes in cold temperature and humidity of fruit and vegetable cold chain during storage and the temperature and humidity during pre-cooling, based on the optimal temperature of different fresh agricultural products during transportation and the existing cold chain transportation mode. A three-level pre-cooling storage mode and a series of full-temperature and wide-width storage cold chain storage and preservation collaborative call module are pro-posed to effectively improve the quality of cold storage and preservation of products, extend their storage period, and improve the storage quality of products. The objective is to expand the efficiency and temperature adaptability of cold chain agricultural products storage quality preservation technology.