THERMAL SCIENCE

International Scientific Journal

Thermal Science - Online First

online first only

The effect of bioresorbable additives and micro-bioobjects on gel formation and stabilization

ABSTRACT
The same properties of agarose gels containing neutral bioresorbable additives and living microorganisms which are important for use in additive technologies of bioreactors creation were considered. Data on the kinetics of gel formation from the solution during cooling were obtained by spectroscopic measurement by measuring the shift of the maximum spectrum of light passing through the gel, depending on the temperature. The dynamics of aging was investigated for gels of different concentrations of agarose, bioresorbable additives and living cells. The time dependences of the decrease in the optical transparency of such gels during the aging process, characterizing the changes in their structure, were obtained. Special attention was paid to the effect of liquid evaporation from gels in the process of gel formation and during long-term storage on relaxation processes leading to their spontaneous increase in density. Experiments were performed to determine the dynamics of the temperature fields simultaneously with heat flux measurements during the formation of studied gels with different concentrations. On the basis of the obtained experimental data and previously developed method, the thermophysical coefficients of agarose gels containing an admixture of starch and living yeast cells were calculated.
KEYWORDS
PAPER SUBMITTED: 2018-12-07
PAPER REVISED: 2018-12-08
PAPER ACCEPTED: 2018-12-09
PUBLISHED ONLINE: 2018-12-16
DOI REFERENCE: https://doi.org/10.2298/TSCI181207350P
REFERENCES
  1. Rodrigues, C. A. V., et al., Stem Cell Cultivation in Bioreactors, Biotechnology Advances, 29 (2011), pp. 815-829
  2. Placzek, M. R., et al., Stem Cell Bioprocessing: Fundamentals and Principles, J. R. Soc. Interface, 6 (2009), pp. 209-232
  3. Ferris, C. J., et al., Biofabrication: An Overview of the Approaches used for Printing of Living Cells, Appl. Microbiol. Biotechnology, 97 ( 2013), pp. 4243−4258
  4. Melchels, F. P. W., et al., Additive Manufacturing of Tissuesand Organs. Prog. Polym. Sci., 37 (2012), pp. 1079−1104.
  5. Marga, F., et al., Toward Engineering Functional Organ Modules by Additive Manufacturing, Biofabrication, 4 ( 2012), ID 02200
  6. Wang, M. Y., et al., The Trend Towards in Vivo Bioprinting, International Journal of Bioprinting, 1 (2015), 1, pp. 15-26
  7. Wang, S., et al., Smart Hydrogels for 3D Bioprinting, International Journal of Bioprinting, 1 (2015), 1, pp. 3-14
  8. Jang, T.-S., et al., 3D Printing of Hydrogel Composite Systems: Recent Advances in Technology for Tissue Engineering. International Journal of Bioprinting, 4 (2018), 126 dx.doi.org/10.18063/IJB.v4i1.126
  9. Bakarich, Sh. E., et al., Three-Dimensional Printing Fiber Reinforced Hydrogel Composites. ACS Appl. Mater. Interfaces, 6 (2014), pp 15998-16006
  10. Kesti, M., et al., A Versatile Bioink for Three-Dimensional Printing of Cellular Scaffolds Based on Thermally and Photo-Triggered Tandem Gelation. Acta Biomaterialia, 11 (2015), pp. 162-172
  11. Rivest, Ch., et al., Microscale Hydrogels for Medicine and Biology: Synthesis, Characteristics and Applications, Journal of Mehanics of Materials and Structures, 2 (2007), 6, pp. 1103-1119
  12. Ozbolat, I.T., et al., Evaluation of Bioprinter Technologies. Additive Manufacturing, 13 (2017), pp. 179-200
  13. Byoung, S.K., et al., Three-Dimensional Bioprinting of Cell-Laden Constructs with Polycaprolactone Protective Layers for Using Various Thermoplastic Polymers. Biofabrication, 8 (2016), 3, 035013
  14. Mineo, W., et al., Agarose Gels: Effect of Sucrose, Glucose, Urea, and Guanidine Hydrochloride on the Rheological and Thermal Properties. J. Agric. Food Chemistry, 38 (1990), 5, pp. 1181-1187
  15. Tuson, H. H., et al., Polyacrylamide Hydrogels as Substrates for Studying Bacteria, Chemical Communications, 48 (2012) pp. 1595-1597
  16. Amsden, B., Solute Diffusion within Hydrogels. Mechanisms and Models, Macromolecules, 31 (1998), pp. 8382-8395
  17. Pokusaev, B., et al., Unsteady Heat and Mass Transfer in Gels, Used as Media for Immobilizing Micro Bio-Objects. MATEC Web of Conferences, 115 (2017), 01001
  18. Lai, M.-F., Lii, C., Rheological and Thermal Characteristics of Gel Structures from Various Agar Fractions. International Journal of Biological Macromolecules, 21 (1997), pp. 123-130
  19. Lahaye, M., Rochas C., Chemical Structure and Physico-Chemical Properties of Agar. Hydrobiologia, 221 (1991), pp. 137-148
  20. Ross, K.A., et al., The Effect of Mixig Conditions on the Material Properties of an Agar Gel - Microstructural and Macrostructural Consideration. Food Hydrocolloids, 20 (2006), pp. 79-87
  21. Medina-Esquivel, R., et al., Measurement of the Sol-Gel Transition Temperature in Agar. Int. J. Thermophys, 29 (2008), 2036. doi.org/10.1007/s10765-007-0332-6
  22. Praiboon, J., et al., Physical and Chemical Characterization of Agar Polysaccharides Extracted from the Thai and Japanese Species of Gracilaria. Science Asia, 32 (2006), 1, pp. 11-17
  23. Pokusaev, B., et al., Laws of the Formation and Diffusion Properties of Silica and Agarose Gels. Theoretical Foundations of Chemical Engineering, 52 (2018), 2, pp. 222-233
  24. Pokusaev, B. G., et al., Non-Stationary Heat Transfer in Gels Applied to Biotechnology. Thermal science, 21 (2017), 5, pp.2237-2246
  25. Somboon, N., et al., Properties of Gels from Mixed Agar and Fish Gelatin. International Food Research Journal, 21 (2014), 2, pp. 485-492
  26. Mishra, G. P., et al., Effect of Hydrophobic and Hydrophilic Additives on Sol-Gel Transition and Release Behavior of Timolol Maleate from Polycaprolactone-Based Hydrogel. Colloid Polym. Sci., 289 (2011), p. 1553, doi.org/10.1007/s00396-011-2476-y
  27. Klouda, L., Mikos, A. G., Thermoresponsive Hydrogels in Biomedical Applications. Eur. J. Pharm. Biopharm., 68 (2008), 1, pp. 34-45
  28. Owens, G. J., et al., Sol-Gel Based Materials for Biomedical Applications. Progress in Materials Science, 77 (2016), pp. 1-79
  29. Patel, H., et al., Biodegradable Polymer Scaffold for Tissue Engineering. Trends Biomater. Artif. Organs, 25 (2011), 1, pp. 20-29
  30. Hutmacher, D. W., et al., An Introduction to Biodegradable Materials for Tissue Engineering Applications. Ann. Acad. Med. Singapore, 30 (2001), 2, pp. 183-191
  31. Lin, S., et al., Influence of Physical Properties of Biomaterials on Cellular Behavior. Pharmaceutical Research, 28 (2011), 6, pp. 1422-1430
  32. Chieh, H.-F., et al., Effects of Cell Concentration and Collagen Concentration on Contraction Kinetics and Mechanical Properties in a Bone Marrow Stromal Cell-Collagen Construct. J. Biomed. Mater. Res. A, 93 (2010), 3, pp. 1132-1139
  33. Buckley, C. T., et al., The Effect of Concentration, Thermal History and Cell Seeding Density on the Initial Mechanical Properties of Agarose Hydrogels. J. Mech. Behav. Biomed. Mater., 2 (2009), 5, pp. 512-521
  34. Peberdy, J. F., Fungal Cell Walls - A Review, in: Biochemistry of Cell Walls and Membranes in Fungi (Eds. P. J. Kuhn, et al.), Springer, Berlin, Heidelberg, 1990, pp. 5-30