A combined technology of high-temperature heating and microwave is proposed to treat bamboo substrates prior to enzymatic hydrolysis to overcome the limits of ei- ther technology. The combined technology helps to promote the reducing sugar yield from enzymatic hydrolysis of bamboo. The protein adsorption test indicated that more enzyme proteins could adsorb on the surface of bamboo pretreated by the combined technology. Thus, the possibility of enzymes to hydrolyze bamboo increased. Meanwhile, X-ray diffractometry tested revealed that the combined technology benefited the removal of non-cellulosic substances from bamboo and resulted in a higher crystallinity.